Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.
Identifieur interne : 001209 ( Main/Exploration ); précédent : 001208; suivant : 001210Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.
Auteurs : Isabel Sospedra [Espagne] ; Carla Soler ; Jordi Ma Es ; José Miguel SorianoSource :
- Journal of chromatography. A [ 1873-3778 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Enterotoxins.
- chemistry : Citrus sinensis, Fruit, Milk.
- methods : Chromatography, Reverse-Phase, Spectrometry, Mass, Electrospray Ionization.
- Animals, Limit of Detection, Linear Models, Reproducibility of Results.
Abstract
Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC-ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples.
DOI: 10.1016/j.chroma.2012.03.022
PubMed: 22498351
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000606
- to stream PubMed, to step Curation: 000606
- to stream PubMed, to step Checkpoint: 000606
- to stream Ncbi, to step Merge: 000F69
- to stream Ncbi, to step Curation: 000F69
- to stream Ncbi, to step Checkpoint: 000F69
- to stream Main, to step Merge: 001221
- to stream Main, to step Curation: 001209
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.</title><author><name sortKey="Sospedra, Isabel" sort="Sospedra, Isabel" uniqKey="Sospedra I" first="Isabel" last="Sospedra">Isabel Sospedra</name><affiliation wicri:level="2"><nlm:affiliation>Department of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. m.isabel.sospedra@uv.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Soler, Carla" sort="Soler, Carla" uniqKey="Soler C" first="Carla" last="Soler">Carla Soler</name></author><author><name sortKey="Ma Es, Jordi" sort="Ma Es, Jordi" uniqKey="Ma Es J" first="Jordi" last="Ma Es">Jordi Ma Es</name></author><author><name sortKey="Soriano, Jose Miguel" sort="Soriano, Jose Miguel" uniqKey="Soriano J" first="José Miguel" last="Soriano">José Miguel Soriano</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22498351</idno><idno type="pmid">22498351</idno><idno type="doi">10.1016/j.chroma.2012.03.022</idno><idno type="wicri:Area/PubMed/Corpus">000606</idno><idno type="wicri:Area/PubMed/Curation">000606</idno><idno type="wicri:Area/PubMed/Checkpoint">000606</idno><idno type="wicri:Area/Ncbi/Merge">000F69</idno><idno type="wicri:Area/Ncbi/Curation">000F69</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F69</idno><idno type="wicri:Area/Main/Merge">001221</idno><idno type="wicri:Area/Main/Curation">001209</idno><idno type="wicri:Area/Main/Exploration">001209</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.</title><author><name sortKey="Sospedra, Isabel" sort="Sospedra, Isabel" uniqKey="Sospedra I" first="Isabel" last="Sospedra">Isabel Sospedra</name><affiliation wicri:level="2"><nlm:affiliation>Department of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. m.isabel.sospedra@uv.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Soler, Carla" sort="Soler, Carla" uniqKey="Soler C" first="Carla" last="Soler">Carla Soler</name></author><author><name sortKey="Ma Es, Jordi" sort="Ma Es, Jordi" uniqKey="Ma Es J" first="Jordi" last="Ma Es">Jordi Ma Es</name></author><author><name sortKey="Soriano, Jose Miguel" sort="Soriano, Jose Miguel" uniqKey="Soriano J" first="José Miguel" last="Soriano">José Miguel Soriano</name></author></analytic><series><title level="j">Journal of chromatography. A</title><idno type="eISSN">1873-3778</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chromatography, Reverse-Phase (methods)</term><term>Citrus sinensis (chemistry)</term><term>Enterotoxins (analysis)</term><term>Fruit (chemistry)</term><term>Limit of Detection</term><term>Linear Models</term><term>Milk (chemistry)</term><term>Reproducibility of Results</term><term>Spectrometry, Mass, Electrospray Ionization (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Enterotoxins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term><term>Milk</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Reverse-Phase</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Limit of Detection</term><term>Linear Models</term><term>Reproducibility of Results</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC-ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><noCountry><name sortKey="Ma Es, Jordi" sort="Ma Es, Jordi" uniqKey="Ma Es J" first="Jordi" last="Ma Es">Jordi Ma Es</name><name sortKey="Soler, Carla" sort="Soler, Carla" uniqKey="Soler C" first="Carla" last="Soler">Carla Soler</name><name sortKey="Soriano, Jose Miguel" sort="Soriano, Jose Miguel" uniqKey="Soriano J" first="José Miguel" last="Soriano">José Miguel Soriano</name></noCountry><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Sospedra, Isabel" sort="Sospedra, Isabel" uniqKey="Sospedra I" first="Isabel" last="Sospedra">Isabel Sospedra</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001209 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001209 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22498351
|texte= Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22498351" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |